Quantitative surface-enhanced resonance Raman scattering of phthalocyanine-labelled oligonucleotides
نویسندگان
چکیده
The evaluation of phthalocyanine labels for the surface-enhanced resonance Raman scattering (SERRS) detection of oligonucleotides is reported. Three phthalocyanine-labelled oligonucleotides were assessed, each containing a different metal centre. Detection limits for each labelled oligonucleotide were determined using two excitation frequencies where possible. Limits of detection as low as 2.8 x 10(-11) mol. dm(-3) were obtained which are comparable to standard fluorescently labelled probes used in previous SERRS studies. The identification of two phthalocyanine-labelled oligonucleotides without separation was also demonstrated indicating their suitability for multiplexing. This study extends the range of labels suitable for quantitative surface-enhanced resonance Raman scattering with silver nanoparticles and offers more flexibility and choice when considering SERRS for quantitative DNA detection.
منابع مشابه
Highly sensitive detection of dye-labelled DNA using nanostructured gold surfaces.
Careful control of surface chemistry results in strong surface enhanced resonance Raman scattering from dye-labelled oligonucleotides assembled on nanostructured gold surfaces, releasing their potential as reliable enhancing surfaces.
متن کاملRaman spectra of zinc phthalocyanine monolayers adsorbed on glassy carbon and gold electrodes by application of a confocal Raman microspectrometer
Raman spectra of zinc phthalocyanine monolayers, adsorbed on gold and on glassy carbon surfaces (electrodes), are presented. These spectra have been recorded with the electrodes inside and outside an electrochemical cell filled with an aqueous electrolyte. A confocal Raman microspectrometer was used. It was demonstrated that, because this spectrometer uses low laser intensities, no damage of th...
متن کاملSurface Enhanced Raman Scattering of Crystal Violet with Low Concentrations Using Self-Assembled Silver and Gold-Silver Core-Shell Nanoparticles
The active substrates in surface enhanced Raman scattering (SERS) spectroscopy were prepared through self-assembly of nanoparticles on functionalized glasses. Colloidal silver nanoparticles (Ag NPs) were prepared chemically in two different sizes by reduction of AgNO3 using trisodium citrate and sodium borohydride. Gold–silver core–shell nanoparticles were also prepared to compare between the o...
متن کاملMultiplexed detection of six labelled oligonucleotides using surface enhanced resonance Raman scattering (SERRS).
The labelling of target biomolecules followed by detection using some form of optical spectroscopy has become common practice to aid in their detection. This approach has allowed the field of bioanalysis to dramatically expand; however, most methods suffer from the lack of the ability to discriminate between the components of a complex mixture. Currently, fluorescence spectroscopy is the method...
متن کاملAdsorption and Surface-Enhanced Raman of Dyes on Silver and Gold Sols'
Several negatively charged dyes were investigated for their possible adsorption on the surface of silver and gold colloidal particles. Those dyes that were found to adsorb on the particles were then checked for surface enhancement of Raman scattering. Highly efficient surface-enhanced Raman scattering (SERS) was observed from a carbocyanine dye in both sols. Excitation-dependence studies as wel...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره 35 شماره
صفحات -
تاریخ انتشار 2007